Project: Metal microdrive and head cap system for silicon probe recovery in freely moving rodent

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Description

High-yield electrophysiological extracellular recording in freely moving rodents provides a unique window into the temporal dynamics of neural circuits. Recording from unrestrained animals is critical to investigate brain activity during natural behaviors. The use and implantation of high-channel-count silicon probes represent the largest cost and experimental complexity associated with such recordings making a recoverable and reusable system desirable. To address this, we have designed and tested a novel 3D printed head-gear system for freely moving mice and rats. The system consists of a recoverable microdrive printed in stainless steel and a plastic head cap system, allowing researchers to reuse the silicon probes with ease, decreasing the effective cost, and the experimental effort and complexity. The cap designs are modular and provide structural protection and electrical shielding to the implanted hardware and electronics. We provide detailed procedural instructions allowing researchers to adapt and flexibly modify the head-gear system.



Animals

Animal name

Investigator

Sessions

Animal actions

DC_M01
Male, Mouse, DBA2/J
Wild type
Mihály Vöröslakos 1 Probe implants
Silicon probe NeuroNexus Buzsaki32 (32 ch, 4 shanks, staggered) | ACA | (1.5,0.75,0.7)
DC_R07
Male, Rat, Long Evans
Wild type
Mihály Vöröslakos 1 Probe implants
Probe Euisik Yoon, University of Michigan Flexible_Polyimide_64ch_poly3 (64 ch, 1 shanks, poly 3 Custom) | CA1 | (-4.8,-4.6,4.5)

Sessions

Sessions

Animal name

Date

Subsessions

DC_M01_20201006, Sorted units
Public session. Webshare, Globus
DC_M01 Homecage_Sleep, Homecage_Sleep
pre_homecage_201006_090834, stimulation_201006_102655, post_homecage_201006_114734
DC_R07_20200703, Sorted units
Public session. Webshare, Globus
DC_R07
DC_R07_20200703_200703_121708, DC_R07_20200703_200703_131748