Details for 20160210

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  • Date & time: 2016-02-10,

  • Animal subject: PV2

  • Investigator: Sam McKenzie

  • Experimenters: Dan English, Sam McKenzie

  • Project: Ground truth cell-types using various opto-tag approaches

  • Data repositories: NYUshare_Datasets, download session from Web share

  • Created: 2019-04-02 14:41:45 by Peter Petersen
    Updated: 2020-06-27 18:45:19 by Peter Petersen ADMIN

  • Notes:
    Optotagging in homecage

Epochs

Epochs Behavioral paradigm Maze type Built maze Manipulation Duration Notes
20160210 Homecage_Sleep Homecage Homecage_Mouse Light stimulation 8884

Extracellular

Equipment

File format

Sampling rate

nSamples

nChannels

Spike groups

Least significant bit

Intan Technologies, RHD2000 USB interface board (256ch) dat 20000 177680000 64 6 0.195

Implanted probes

PV2 : Silicon probe NeuroNexus Buzsaki 5×12 (64 ch, 5 shanks, staggered Non-uniform) | Region: HIP | Coordinates: , ,

Spike groups

Spike groups are typically defined by the group of channels within shanks. Channels are listed and comma-separated and labels can further be assigned to each group. Channels are 0-indexed.

Group

Channels

Label

1 51, 58, 52, 57, 49, 60, 50, 59, 54, 55, 53, 56
2 63, 38, 64, 39, 61, 41, 62, 42, 33, 37, 34, 35
3 47, 17, 46, 20, 45, 19, 43, 21, 48, 18, 44, 22
4 28, 1, 25, 2, 23, 3, 24, 4, 27, 31, 29, 32
5 8, 13, 7, 14, 6, 15, 5, 16, 9, 12, 10, 11
6 30, 36, 26, 40
This section is dedicated to extra inputs besides the primary recording channels. For extracellular recordings this includes any extra analog and digital channels. Further it can be inputs saved with an Arduino or Raspberry pi or an entirely different hardware solution.

Equipment

Input type

Channel

Tag

Description

Intan Technologies, RHD2000 USB interface board (256ch) adc 0 StimShank1
Intan Technologies, RHD2000 USB interface board (256ch) adc 1 StimShank1
2 StimShank3
3 StimShank4

Equipment

File names

Epoch

Type

Framerate

Notes


Brain regions are defined according to the Allen Institute Atlas. A brain region can be assigned to a list of channels or spike groups.

Brain region

Channels

Spike groups

HIP – Hippocampal region (View in Atlas) 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64

Channel tags

Examples and reserved tags: Bad, Good, Theta, Gamma, Delta, Ripple, RippleNoise.

Tag

Channels

Spike groups

Ripple 56 1
Theta 56 1

Analysis tags

Tag

Value

Description


Spike sorting

Method

Format

Relative path

Channels

Spike sorter

Notes

Sorting completed

Units count

Sorting ID

Klustakwik Neurosuite Sam McKenzie Yes 15 28096

Spike sorted units

# Spike count Firing rate (Hz) Spike group Brain region Cell type Deep/Superficial Refractory violation (‰) Peak voltage (µV) Trough-to-Peak (ms) Isolation distance
1 1686 0.19141882248189 5 HIP Pyramidal Cell Superficial 0 142.18754554826 0.62 0
2 11278 1.2708820095811 4 HIP Pyramidal Cell Superficial 1.2413548501507 167.97338413812 0.59 0
3 11870 1.3364427977488 3 HIP Pyramidal Cell Superficial 8.5088458298231 202.36157257184 0.68 0
4 3191 0.36021287282618 3 HIP Pyramidal Cell Superficial 0 202.40770590952 0.6 69.56558138877
5 3762 0.42549568083711 3 HIP Pyramidal Cell Superficial 0.79744816586922 188.71636832115 0.7 64.333201818695
6 3973 0.44780192822406 3 HIP Wide Interneuron Superficial 0 199.68128933076 0.57 101.32318263485
7 4922 0.55419896411634 2 HIP Pyramidal Cell Superficial 4.2665583096302 254.95332970209 0.7 47.064903473271
8 1177 0.13285371824175 2 HIP Pyramidal Cell Superficial 3.3984706881903 280.21135312771 0.69 31.24621763519
9 4845 0.54551334973626 2 HIP Pyramidal Cell Superficial 1.4447884416925 163.29527680448 0.62 69.755655445195
10 2202 0.24885298353961 2 HIP Pyramidal Cell Superficial 0 216.41190981466 0.64 94.747152913431
11 8697 0.979090264833 2 HIP Pyramidal Cell Superficial 4.9442336437852 194.4105043531 0.6 173.99648790775
12 3337 0.37657497163909 2 HIP Pyramidal Cell Superficial 3.8957147138148 286.55305275611 0.64 60.973074105559
13 28954 3.2615804961902 2 HIP Pyramidal Cell Superficial 4.6625682116461 219.4871378186 0.62 0
14 70984 7.9906607520197 1 HIP Narrow Interneuron Deep 1.0706638115632 245.94537117338 0.2 0
15 9193 1.0353216310042 1 HIP Pyramidal Cell Deep 1.4141194387034 311.74408632768 0.68 399.50195929385